I am currently writing this at 2:32 AM in the morning the day of a AB Calculus test, Biology test and Spanish quiz, because I am stupid and procrastinated on this lab report.
PROCEDURE AND DOCUMENTS
I have included the procedure above for reference
This is my Lab Worksheet that you can reference
For this lab the main "ingredient" was the enzyme we used, which we got from a chicken liver. The basic run down of the procedure is: Put Hydrogen Peroxide in beaker Soak dots in enzyme Put dots in beaker without touching the peroxide Put a stopper over the beaker and put a tube filled with water over the tube from the stopper Put the beaker underwater and turn it upside down so the enzyme and the peroxide mix
In this section I will go through the science behind this experiment. Our group was only able to get two parts down so we were not very efficient but thanks to our teacher we understand what would have happened. Enzymes are proteins lower the activation energy for a chemical reaction and therefore increases the speed of the reaction. We have a bunch of enzymes in our body and we would not survive without them.
Our teacher taught us a great example to understand how enzymes work and the parameters and the conditions: Nate is person (enzyme); People are element; Nate goes around punches people but he has a rate of ten people an hour If you have 10 Nates or ten enzymes there are more punches and more people get punched so it will go faster If there are 200 people and a thousand Nates than you will run out, so to many enzymes will have a cap point You can heat up the temperature to a 1000 degrees and Nate dies and the reaction is broken or you can lower the temperature. Nate only works with a specific temperature and he slower at some temperature. (tempreture) You can put Nate in an acid bath and he will slow down (pH) You can put a bunch of fake dummy Akash's to take punches (Dilute the solution)
The conditions for enzymes vary depending on the enzyme because they are all built for different chemical reactions. Proper amount of enzyme, proper supply of element, proper temperature, pH and if there is any buffer or not, all affect the conditions for enzyme action.
When Hydrogen Peroxide and the enzyme mix, oxygen is produced. We measure that oxygen over time intervals and find the rate of the reaction.
ANALYSIS AND DATA
We had some analysis questions so I will put them here along with my data
ANALYSIS QUESTIONS IN THE SLIDESHOW
TABLE IN THE SLIDESHOW
I thought that this was a very fun lab and I learned a lot about enzymes. I only wish that I had more time because we only got through 2 runs which were hurried up. We also made a few mistakes and did not hold the tube properly so that also sucked. My lab group had a lot of fun and we all participated.